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Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 9: Construct-specific real-time PCR based screening method for the detection of the P35S-nptII DNA-sequence
| 분야 | ISO/TC 34/SC 16 : Horizontal methods for molecular biomarker analysis |
|---|---|
| 적용범위 | This proposed Technical Specification describes a procedure for the detection of the DNA transition sequence between the 35S promoter region from Cauliflower mosaic virus (P-35S) and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli. The P-35S-nptII construct which confers resistance to neomycin/kanamycin antibiotics is frequently found in genetically modified plants. The detection method is based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out. This part of ISO 21569 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix. |
| 국제분류(ICS)코드 | 67.050 : 식품 산업의 시험 및 분석의 일반적 방법 |
| 페이지수 | 10 |
| Edition | 1 |
| No. | 표준번호 | 표준명 | 발행일 | 상태 |
|---|---|---|---|---|
| 1 | ISO/DTS 21569-9 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 9: Construct-specific real-time PCR based screening method for the detection of the P35S-nptII DNA-sequence | 초안 |
ISO/DTS 21569-8 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 8: DNA extraction from alfalfa seeds and real-time PCR based detection methods for genetically modified alfalfa events J101, J163 and KK179 상세보기
ISO/TS 21569-6:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences 상세보기
ISO/TS 21569-4:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences 상세보기
ISO/TS 21569-5:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence 상세보기
ISO/TS 21569-3:2020 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms 상세보기
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