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Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 8: DNA extraction from alfalfa seeds and real-time PCR based detection methods for genetically modified alfalfa events J101, J163 and KK179
| 분야 | ISO/TC 34/SC 16 : Horizontal methods for molecular biomarker analysis |
|---|---|
| 적용범위 | This Technical Specification describes procedures for DNA extraction from alfalfa seeds and for the event-specific detection of the alfalfa lines J101, J163 and KK179. The detection methods are based on real-time PCR and are targeting the DNA transition sequences between the alfalfa genome and the respective integrated gene construct. The methods can be applied for direct event-specific identification or as a follow-up analysis, if sequences encoding the promoter of the figwort mosaic virus (P-FMV), the terminator of the nopaline synthase gene from Rhizobium radiobacter (T-nos), or the construct CTP2-CP4-EPSPS (herbicide tolerance) were detected by screening analyses of alfalfa samples. In this part of ISO 21569 the DNA extraction method was validated using ground alfalfa seeds and the PCR methods using DNA extracted from seeds. The PCR methods may also be used for the analysis of other matrices (e.g. feed- and foodstuffs). The application of the PCR methods require the extraction of an adequate amount of amplifiable DNA from the relevant matrix. |
| 국제분류(ICS)코드 | 67.050 : 식품 산업의 시험 및 분석의 일반적 방법 |
| 페이지수 | 12 |
| Edition | 1 |
| No. | 표준번호 | 표준명 | 발행일 | 상태 |
|---|---|---|---|---|
| 1 | ISO/DTS 21569-8 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 8: DNA extraction from alfalfa seeds and real-time PCR based detection methods for genetically modified alfalfa events J101, J163 and KK179 | 초안 |
ISO/DTS 21569-9 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 9: Construct-specific real-time PCR based screening method for the detection of the P35S-nptII DNA-sequence 상세보기
ISO/TS 21569-4:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences 상세보기
ISO/TS 21569-6:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences 상세보기
ISO/TS 21569-5:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence 상세보기
ISO/TS 21569-3:2020 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms 상세보기
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