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Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences
| 분야 | ISO/TC 34/SC 16 : Horizontal methods for molecular biomarker analysis |
|---|---|
| 적용범위 | ISO/TS 21569-4:2016 specifies a procedure for the detection of a DNA sequence of the promoter region of the nopaline synthase gene (P-nos) from Agrobacterium tumefaciens and a procedure for the detection of the DNA transition sequence between P-nos and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli K12. The nos-promoter and the P-nos-nptII-construct are frequently found in genetically modified plants. The P-nos and P-nos-nptII specific methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out. The methods described are applicable for the analysis of DNA extracted from foodstuffs. They may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix. The DNA sequence amplified by the P-nos element-specific method can be detected in samples which contain DNA of the naturally occurring Ti-plasmid of A. tumefaciens. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods. |
| 국제분류(ICS)코드 | 67.050 : 식품 산업의 시험 및 분석의 일반적 방법 |
| 페이지수 | 10 |
| Edition | 1 |
| No. | 표준번호 | 표준명 | 발행일 | 상태 |
|---|---|---|---|---|
| 1 | ISO/TS 21569-4:2016 | Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences | 2016-11-02 | 표준 |
ISO/DTS 21569-8 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 8: DNA extraction from alfalfa seeds and real-time PCR based detection methods for genetically modified alfalfa events J101, J163 and KK179 상세보기
ISO/DTS 21569-9 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 9: Construct-specific real-time PCR based screening method for the detection of the P35S-nptII DNA-sequence 상세보기
ISO/TS 21569-6:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences 상세보기
ISO/TS 21569-5:2016 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence 상세보기
ISO/TS 21569-3:2020 - Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms 상세보기
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ISO 5526:2013 - Cereals, pulses and other food grains — Nomenclature 상세보기
ISO 5536:2009 - Milk fat products — Determination of water content — Karl Fischer method 상세보기
ISO 24276:2006 - Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — General requirements and definitions 상세보기
ISO 21571:2005 - Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction 상세보기
IEC TS 63134:2020 - Active assisted living (AAL) use cases 상세보기
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